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KMID : 1129720200370020097
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Korean Journal of Acupuncture
2020 Volume.37 No. 2 p.97 ~ p.103
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Neuroprotective Effects of Cervi Cornu in MPP+ Treated SH-SY5Y Cells
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Yeo Su-Jung
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Abstract
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Objectives: : Parkinson’s disease, a progressive neurodegenerative disease, is caused by the loss of dopaminergic neurons in the substantia nigra. There is no clear treatment or remedy for Parkinson’s disease; therefore, the development of novel therapies related to anti-inflammatory and antioxidant effects is required. This study was performed to evaluate the neuroprotective effect of water extracts from Cervi Cornu (CC) in dopaminergic cells.
Methods : We studied effects of CC on apoptosis, cell death and inflammation in SH?SY5Y neuroblastoma cells treated by methylpyridinium ion (MPP+). SH-SY5Y cell line was treated with CC for 24 hours and then 500 μM MPP+ for 18 hours.
Results : Cervi Cornu treatment inhibited the decrease in tyrosine hydroxylase (TH) expression and decreased the activation of inflammatory factors mitochondrial cytochrome C oxidase (COX2) and inducible NO synthase (iNOS) against MPP+ neurotoxicity. Apoptosis factors BCL2 associated X, apoptosis regulator (BAX) levels were decreased and B-Cell CLL/Lymphoma 2 (BCL2) levels were increased.
Conclusions : These results suggest that CC treatment had neuroprotective effects in the SH?SY5Y neuroblastoma cells against toxicity induced by MPP+. The results suggest new possibilities of CC for the treatment of Parkinson’s disease.
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KEYWORD
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Cervi Cornu, Parkinson’s disease, neuroprotection, MPP+, SH?SY5Y
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