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KMID : 1145320120010010053
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Journal of Korea Immuno-yakchim Society
2012 Volume.1 No. 1 p.53 ~ p.62
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Antioxidant, Anti-inflammatory activities and Inhibition Effect on the Inflammtory cytokines of CS Yakchim Fluid
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Kim Hak-Ju
Choi Il-Whan
Jung Chul
Lim Hyung-Ho
Song Yun-Kyung
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Abstract
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Objectives: This purpose of this study was to analyzed the Biological Activities of CA Yakchim Fluid.
Methods: The protein content, glucose content, crude fat content, crude ash content in CA Yachim fluid were analyzed, and DPPH radical scavenging, inhibition of COX-2 activities were investigated. Also TNF-α, IL-6, GAPDH as inflammatory cytokines were measured.
Results: The protein, 1.46%, glucose, 0.13%, crude fat 96.18%, crude ash 0.51% in CA Yachim fluid were confirmed. The electron donation ability of CA Yakchim on DPPH WAS 21%. The inhibition of COX-2 activity of CA Yakchim fluid was 68%. The TNF-α, IL-6 were increased in RAW 264.7 treated with LPS, and were decreased in RAW 264.7 pre-treated with CA Yakchim fluid.
Conclusions: We conclude that the CA Yakchim fluid has anti-oxidant, anti-inflammatory activities and inhibition effect of the inflammatory cytokines.
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KEYWORD
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CA Yakchim, anti-oxidant, anti-inflammatory activities, cytokines, Nam Sang Cheon, Immuno Yakchim, Pharmacopuncture
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