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KMID : 1145320150040010019
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Journal of Korea Immuno-yakchim Society
2015 Volume.4 No. 1 p.19 ~ p.24
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In Vivo Micronucleus Test of CSⅡ-YAKCHIM in Mice
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Lee Ma-Seong
Jeong Jin-Ho
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Abstract
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Objectives : This study was designed to determine the potential of CSⅡ-YAKCHIM, manufactured from Brassicae Semen, Raphani Semen, Persicae Semen, and Carthami Fructus extracts, to induce micronuclei in bone marrow cells of mice when the CSⅡ-YAKCHIM was dosed once to ICR mice by intramuscular.
Methods : Each of the groups consisted of three doses of CSⅡ-YAKCHIM (0.05, 0.1, and 0.2 mL/animal), positive control (Mitomycin C), and negative control (normal saline). The time to harvest bone marrow cells for the main study was 24 hours.
Results : The incidence of micronucleated polychromatic erythrocyte (MNPCE) among polychromatic erythrocyte (PCE) was not significantly increased in the CSⅡ-YAKCHIM-treated groups. Also, the ratio of PCE among total erythrocyte of the CSⅡ-YAKCHIM-treated groups was not significantly different when compared to that of the negative control group.
Conclusions : Based on these results, CSⅡ-YAKCHIM did not have any potential to induce micronuclei formation in bone marrow cells of mice under the conditions of this study
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KEYWORD
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CSⅡ-YAKCHIM, pharmacopuncture, toxicity, micronucleus test, Brassicae Semen, Raphani Semen, Persicae Semen, Carthami Fructus
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