KMID : 1147720100030030150
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Journal of Acupuncture and Meridian Studies 2010 Volume.3 No. 3 p.150 ~ p.155
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Fluorescent Nanoparticles for Observing Primo Vascular System Along Sciatic Nerve
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Jia Zhao-Feng
Lee Byung-Cheon Eom Ki-Hoon Cha Jin-Myung Lee Jin-Kyu Su Zhen-Dong Yu Wen-Hui Ryu Pan-Dong Soh Kwang-Sup
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Abstract
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The primo vascular system was found in the epineurium along the rat sciatic nerve following subcutaneous injection of fluorescent nanoparticles at the Zusanli acupoint (ST-36). Nanoparticles were injected into the primo-vessel near ST-36 and flowed along the sciatic nerve. Fluorescence revealed a structure in the epineurium that was hardly detectable. Images of the isolated sample stained with 4¡Ç,6-diamidino-2-phenylindole were captured using confocal microscopy. These images showed the distinctive nuclei distribution and multi-lumen structure of primo-vessels that differentiate them from lymphatic vessels, blood capillaries and nerves. This study demonstrates a new use for nanoparticles in fluorescence reflectance imaging techniques during in vivo imaging of primo-vessels.
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KEYWORD
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acupuncture, epineurium, primo vascular system, primo-vessel, sciatic nerve, Zusanli (ST-36)
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