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KMID : 1189220090360030175
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Korean Journal of Reproductive Medicine
2009 Volume.36 No. 3 p.175 ~ p.186
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The effect of low concentrated hypoxanthine and FSH in 10% FBS supplemented medium on immature oocyte in vitro maturation
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Han Hyuk-Dong
Lim Chang-Kyo
Youm Hyun-Sik
Hyon Naomi Nahyoung
Lee Ji-Hyang
Hong Mi
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Abstract
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Objective: We examined the effect of different culture media on oocyte maturation.
Methods: Four groups of media, ① 0.3% BSA mBASAL-XI-HTF, ② 0.3% BSA mBASAL-XI-HTF with FSH, ③ 10% FBS mBASAL-XI-HTF and ④ 10% FBS mBASAL-XI-HTF with FSH were prepared. Mouse cumulus enclosed oocytes (CEOs) were incubated in each group of medium. Hypoxanthine (Hx) was mixed to each group of medium in increasing concentrations of 1 mM, 2 mM and 4 mM. CEOs were incubated and assessed for GVBD and MII development at 3, 6, 18 hours.
Results: CEOs maturation to GVBD was seen in all four groups during 3 hours of culture, however MII stage of oocytes was seen after 6 hours. Complete arrest of GV stage in 4 mM Hx media without FSH and partial arrest in 2 mM Hx media without FSH were seen during 18hours of culture but development was not suppressed in 1 mM Hx media without FSH. More prominent GVBD suppression was noted at early 3, 6 hours culture in 1 mM, 2 mM Hx media with FSH compared to media without FSH. But the suppression was recovered at 18 hours. This result suggests that low concentrated Hx and FSH supplemented media can suppress CEOs maturation during early culture period but recovery is resumed or even stimulated at late period. 1 mM, 2 mM Hx 10% FBS medium with FSH had significantly higher rates of MII development (71.7%, 66.7%) at 18 hours compared to other media.
Conclusion: Our results show that low concentrated Hx and FSH supplemented 10% FBS media may stimulate MII development after an initial inhibitory effect.
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KEYWORD
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Immature oocyte, In vitro maturation, Hypoxanthine, FSH
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