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KMID : 1189220100370030207
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Korean Journal of Reproductive Medicine
2010 Volume.37 No. 3 p.207 ~ p.216
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Role of HOXA Gene in Human Endometrial Decidualization
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Park Dong-Wook
Lee Chang-Se
Park Chan-Woo
Kim Tae-Jin
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Abstract
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Objective: This study was performed to clarify the role of HomeoboxA (HOXA) and its related signaling molecules in the decidualization of primary cultured endometrial cells.
Methods: Human endometrial tissues were obtained by curettage of hysterectomy specimens from patients with conditions other than endometrial diseases. Tissues were minced and digested with Trypsin-EDTA for 20 min, 37℃. Cells were cultured with DMEM/F12 medium in 37℃, 5% CO2 incubator for 24 hrs. Cells were treated with HOXA10 siRNA and added transforming growth factor (TGF)-β1 (10 ng/mL) for 48 hrs to induces decidualization in vitro. Reverse transcription polymerase chain reaction analysis was accomplished to observe the expression of HOXA10, prolactin, cyclooxygenase (COX)-2, peroxisome proliferator￢activated receptor (PPAR)-γ, and wingless-type MMTV integration site family (Wnt).
Results: HOXA10 expression was increased (1.8 fold vs. non-treated control) in TGF-β1 treated cells. Decidualization marker, prolactin, was significantly increased in TGF-β1 treated cells compared with HOXA10 siRNA treated cells. Endometrial cell differentiation marker, COX-2 was down-regulated by HOXA10 siRNA even if cells were treated with TGF-β1. Wnt4 was down-regulated by treated with HOXA10 siRNA, this expression patters was not changed by TGF-β1. Expression of PPAR-γ was down regulated by TGF-β1 in regardless of HOXA10 siRNA treatment.
Conclusion: TGF-β1 which is induced by progesterone in endometrial epithelial cells may induces stromal cell decidualization via HOXA10 and Wnt signaling cascade.
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KEYWORD
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HOXA, Wnt, Decidualization, Endometrium
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