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KMID : 1235920090010010030
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Medical Journal of Catholoc University of Daegu
2009 Volume.1 No. 1 p.30 ~ p.37
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Association of Estrogen Receptor-αgene Expression with Bone Density in Postmenopausal Women
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Min Kang-Woo
Moon Yong-Suk
Kim Hong-Tae
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Abstract
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Background: Estrogen plays an important role in maintaining bone mass, and postmenopausal osteoporosis is clearly linked to its deprivation. Estorgen receptor-α(ERα) is present in human osteoblast and mediates osteogenic effect of estrogen on bone. However, the precise role that estrogen and ERαplay in the regulation of osteoblastic function in
postmenopausal women with ostoeporosis is not clear.
Methods: To evaluate the correlations between the bone density (BMD) measured by X-ray absorptiometry (DXA) and ERαgene expression in osteoblasts derived from postmenopausal women, we performed histomorphometric analysis, immunohistochemistry and RT-PCR with bone biopsies obtained from 23 women who underwent knee joint replacement.
Results: The correlations between BMD and the area of bony trabeculae measured in the size of 25 mm2 histological sections was positive (r2=0.46, p<0.05) but there was no correlation between BMD and the ERαgene expression in human osteoblasts. Image analysis showed that proportions of osteoblasts positive for immunoreactable ERαwere not different in women with osteoporosis or osteopenia (mean±SD; 54.6±15.1%, 54.8±11.5%, respectively). Although the relative expression levels of F-hERαmRNA in bone biopsies of ostoeoporotic women(1.3±0.5) was relatively
higher than in those of osteopenic women (0.79±0.64), this was not statistically significant. The ratio of expression of two F-hERαvariants hERα66 and hERα46 in bone biopsies of each group was not different.
Conclusion: In summary, the expression of ERαgene in osteoblasts of postmenopausal women was maintained an even level without the relevance of the BMD. This study indicates that ERαcould play a important role in maintaining bone mass and bone formation in postmenopausal women.
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KEYWORD
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Estrogen receptor-α, Bone density, Osteoporosis, F-hER αvariant
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