KMID : 1235920090010010038
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Medical Journal of Catholoc University of Daegu 2009 Volume.1 No. 1 p.38 ~ p.45
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Immunohistochemical Studies of Regeneration in the Rat Olfacgtory Epithelium after Formaldehyde Inhalation
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Moon Yong-Suk
Kim Hong-Tae
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Abstract
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The present study was carried out to investigate the properties of regeneration of the olfactory epithelium in the rat after formaldehyde inhalation. Sprague-Dawley male rats were inhalated 30 ppm formaldehyde for 3 times with 3 hours exposure. The olfactory mucosa were taken from the animals on 3, 6, 9 days and 2, 3, 4, 5 weeks after formaldehyde inhalation. The mitotic activity of the olfactory epithelial cells were investigated using bandeiraea simplicifolia lectin I (BSL I), phaseolus vulgaris leucoagglutinin (PHA-L) and 5¡¯-bromodeoxyuridine (BrdU) which injected 6 hours before sacrifice of the animals. In experimental groups, the degenerative changes of the olfactory epithelium were observed until 2 weeks after formaldehyde inhalation. The number of BrdU labelled cells in the olfactory epithelium was 17.8¡¾1.50/mm in the control group. The mitotic activities were decreased to 5.5¡¾0.46/mm in 2 weeks and recovered in 3 weeks after formaldehyde inhalation. To find the stem cells of olfactory receptor neurons, double labelling method was performed with BSL I or PHA-L lectin which were specific for horizontal basal cells and BrdU immunohistochemistry. The ratio of globose/horizontal basal cells in BrdU labelled cells with BSL I or PHA-L lectin positive reaction was 85.4%/14.6% in control group, 39.4%/60.6% in lesion group and 58.9%/41.1% in recovery group. These results suggest that the stem cells of olfactory receptor neurons in regeneration of the olfactory epithelium after formaldehyde inhalation are horizontal basal cells.
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KEYWORD
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Olfactory epithelium, Formaldehyde, Regeneration, Stem cells, BrdU
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