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KMID : 1235920090010010086
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Medical Journal of Catholoc University of Daegu
2009 Volume.1 No. 1 p.86 ~ p.89
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Effect of PGF3α on the Survival and Production of Nitric Oxide in Cultured Trabecular Meshwork Cells
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Kim Jae-Woo
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Abstract
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Purpose: To investigate whether prostaglandin F2α(PGF2α) affect on the survival and production of nitric oxide
(NO) in cultured human trabecular meshwork (TM) cells.
Methods: Primarily cultured human TM cells were exposed to 0, 100, 200, 500 nm PGF2α(PhXA85, latanoprost free acid) using serum-deprived media for 3 days to evaluate the effect on the cellular survival and nitric oxide production assessed by MTT and Griess assay, respectively. Also evaluated was in hypoxic conditions induced by sodium cyanide.
Result: PGF2αsignificantly decreased cellular survival of cultured TM cells in a dose-dependent manner accompanied with decreased NO production. PGF2αwas not cytoprotective in hypoxic conditions.
Conclusion: The current results suggest that PGF2αdecreased cellular survival and production of NO. This PGF2α-induced decreased NO production in TM cells may be involved in the down-regulation of trabecular outflow.
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KEYWORD
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Nitric oxide, PGF2α, Trabecular meshwork cells
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