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KMID : 1235920090010020175
Medical Journal of Catholoc University of Daegu
2009 Volume.1 No. 2 p.175 ~ p.180
Mechanism of Non-coplanar PCBs in Developing rat Neurons in Culture
Yang Jae-Ho

Abstract
PCB is widely used as a flame retardant and rises as a major class of environmental pollutants that bioaccumulate in humans. A major route of exposure to this class of chemical is thru food consumption. Since high concentrations of PCB were detected in human breast milk, neonates are considered a high risk group. In particular, non-coplanar PCBs are known to be sensitive to developing brain and associated with neurobehavioral abnormally. The present study attempted to identify molecular target of non-coplanar PCB in developing neuronal cells and understand the mechanism of action. Neurotoxic effects of PCB are related with the function of cerebellum, thus, we used the cerebellar granule cells that can mimic PND-14 conditions. PCB congeners highly detected in human serum and human milk were compared with TCDF. Among PCB congeners tested, PCB-4(2,2¡¯-PCB) was most neuroactive. This congener induced [3H] PDBu binding activity to a level similar to Aroclor1260. PCB-4 and PCB-8 induced a translocation of PKC-¥á and -¥å, as with TCDF or Aroclor1260. Production of ROS was similar between Aroclor1260 and PCB-4. PKC activity and ROS production were similar between Aroclor1260 and PCB-4. PCB-4 only showed high levels of RC-3 mRNA and PCB-15 showed highest level of GAP-43 mRNAs. The present results suggest that neuroactive effects of non-coplanar PCB such as PCB-4 may be higher than that of dioxin-like PCBs. This study demonstrated possible molecular targets of non-coplanar PCB in the developing neurons, which can be utilized as biomarker for risk assessment. In addition, neuronal cell used in the study may provide a valuable tool to ascertain neurotoxic materials from a variety of environmental mixture samples.
KEYWORD
PCB, Non-coplanar, Cerebellar cell, Neuroactive
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