KMID : 1235920090010020211
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Medical Journal of Catholoc University of Daegu 2009 Volume.1 No. 2 p.211 ~ p.214
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Effect of Brimonidine on the Survival and Production of Nitric Oxide in Cultured Trabecular Meshwork Cells
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Kim Jae-Woo
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Abstract
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Purpose: To investigate whether brimonidine affect on the survival and production of nitric oxide (NO) in cultured
human trabecular meshwork (TM) cells.
Material and Methods: Primarily cultured human TM cells were exposed to 0, 10, 50, 100 ¥ìM brimonidine using 1% serum-containing media for 1 day and 3 days. The cellular survival and nitrite production were assessed with MTT and Griess assay, respectively.
Results: Cellular survival of cultured TM cells was significantly decreased after exposure for 1 day and 3 days to 100¥ìM brimonidine. Production of NO was decreased after exposure for 1 day and 3 days to 50 ¥ìM and 100 ¥ìM brimonidine, respectively. Production of NO was decreased after exposure for 3 days to 100 ¥ìM brimonidine.
Conclusion: The current results suggest that brimonidine decreased cellular survival and production of NO. Thus, brimonidine could decrease aqueous outflow through trabecular meshwork.
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KEYWORD
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Brimonidine, Nitric oxidel, Trabecular meshwork cells
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