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KMID : 1235920100020010027
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Medical Journal of Catholoc University of Daegu
2010 Volume.2 No. 1 p.27 ~ p.32
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Comparative Analysis of Synchrotron Radiation Imaging and Histopathologic findings for Fibrocystic Changes in Breast Tissue
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Lee Ja-Hyun
Bong Jin-Gu
Park Sung-Hwan
Kim Jong-Ki
Youn Hwa-Shik
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Abstract
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Purpose: Synchrotron radiation is expected to improve the quality of clinical breast radiography. It also enables
observation of detailed internal structures of breast tissue samples at high magnification with excellent resolution.
The high-resolution synchrotron images of the fibrocystic changes in breast tissue, which are obtained using the
phase contrast technique were compared with the optical microscopic findings.
Materials and Methods: A new X-ray microscope was installed on a 1B2 beamline of a Pohang Light Source (Pohang, Korea), a 3rd generation synchrotron radiation facility with an operating energy of 2.5 GeV. The X-ray energy was set at 11.1 keV, and the X-ray beam was monochromatized using a W/B4C monochromator. A Zernike phase-shifter was adapted for conducting phase contrast X-ray microscopy. Formalin-fixed 10 μm-thick female breast tissue specimens taken from cases of fibrocystic changes were attached onto Kapton film. The X-ray image of the sample was converted into a visual image on the CsI (TI) scintillation crystal. After magnification, this visual image was captured using a full frame charge-coupled device (CCD) camera. The adjacent tissue sections were stained with hematoxylin and eosin and observed by light microscope for conducting a comparative analysis with the synchrotron images.
Results: X-ray microscopic images were obtained with good resolution. This synchrotron radiation imaging enabled us to observe the detailed microscopic structures of the breast tissue. The images showed excellent correspondence with the adjacent stained tissue sections and exhibited findings characteristic of fibrocystic change in breast tissues.
Conclusion: Using synchrotron radiation, X-ray microscopic images of the fibrocystic changes in breast tissue
revealed various histopathologic structures with good contrast and visibility by employing the phase contrast
technique. According to these obtained images, we can tell that X-ray microscopic imaging of breast tissue with
synchrotron radiation has a great potential for use in various clinical and research applications.
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KEYWORD
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Breast, Fibrocystic change, X-ray Microscopy, Synchrotron
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