|
KMID : 1235920100020020060
|
|
Medical Journal of Catholoc University of Daegu
2010 Volume.2 No. 2 p.60 ~ p.67
|
|
|
Isolation and Cytogenetic Analysis of Multiple Clones from a Single Gastric Cancer Cell Line
|
|
Kim Hong-Tae
|
|
|
Abstract
|
|
|
Background: In many human cancers, the cell population within a single neoplasm is often heterogeneous. Establishing in vitro cell lines derived from human cancers provides a model for the study of tumor progression and heterogeneity. The KUMAII cell line was established from a adenocarcinoma of stomach, and its in vitro karyotypic evolution was analyzed. Initially, considerable genetic heterogeneity was evident. The aim of this study is to investigate the extent of cellular diversity within KUMAII cell line by attempting to isolate further cell types and characterize these cell populations.
Materials and Methods: To isolate subclones from KUMAII cell line, we taken frozen stocked 30th passage cells and recultivated. Eight subclones (C-1~C-8) were established from KUMAII cell line by limit dilution and ring colonization methods and have been extensively characterized.
Results: Individual subclones showed a unique set of morphological and cytogenetic characteristic in vitro. The four subclones were hyperdiploidy and others were tetraploidy. Several structural chromosomal anomalies (1p13-, 1p22-, 3q+, 5p13-, 7p+, 8p+, 9p+, 9p-, 15p+, 18q-, 20p+) were commonly appeared in all of subclones. However, each subclone has characteristic abnormal chromosome patterns also. The structural chromosomal anomalies of chromosome 1, 7 and 11 appeared differently in each subclone.
Conclusion: KUMAII cell line and its eight subclones are considered to be a very useful model in study of cell biology and in the development of new therapeutic modalities.
|
|
|
KEYWORD
|
|
|
Cancer cell line, Gastric cancer, Cancer cytogenetics, KUMAII cell line
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|