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KMID : 0043319940170040231
Archives of Pharmacal Research
1994 Volume.17 No. 4 p.231 ~ p.235
Fluorometric Determination of Gastric Acidity with 2-Fluorenecarboxaldehyde Hydrazone
Choi Kyong

Choi Jung-Kap
Yoo Gyurng-Soo
Abstract
Fluorenecarboxaldehyde hydrazone 9FCH)was synthesized as a new fluorescent reagent for the determination of acidity. FCH had no fluorescence in itself, however, it strongly fluoresced under acidic conditions at excitation maximum of 392 nm and emission maximum of 447 nm, respectively. It showed good correlation with pH in the range from pH 0.60 to pH 3.60 in strong acids. As an application, the acidity of gastric juice in rats and humans was determined. In comparision with pH-metry, the acidity measured by the developed method showed generally increased values of about 0.7-1.3 unit and 0.8-1.2 unit in rats and humans, respectively. However, these results had statistically close correlation with those of pH-metry and the correlation coefficients were 0.887 in rats and humans between two methods.
KEYWORD
Gastric juice, acidity, pH, Flouorometic assay, 2-Flourenecarboxaldehyde hydazone(FCH)
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