|
KMID : 0043319940170050343
|
|
Archives of Pharmacal Research
1994 Volume.17 No. 5 p.343 ~ p.347
|
|
|
Betaine Attenuates Glutamate-induced Neurotoxicity in Primary Cultured Brain Cells
|
|
Park Mi-Jung
Kim So-Ra
Huh Hoon
Jung Jee-Hyung
Kim Young-Choong
|
|
|
Abstract
|
|
|
|
Effects of betaine on glutamate-induced neurotoxicity were examined on primary culturs of chicken embryonic brain cells and on rat cortical cultures. Betaine was found to attenuate glutamate-induced neurotoxicity both morphologically and biochemically. A 30 min exposure of chicken embryonic brain cells cultured for 12 days to 500 .mu.M glutamate produced wide-spread acute neuronal swelling and neurtic fragmentation. A 2-h pretreatment of cultured chicken embryonic brain cells with i mM betaine prior to a 30 min exposure to 500 , mu, M glutamate significantly raised the survival rate of neurons in the culture. When chicken embryonic brain cells were pretreated for 2 h with i mM betaine followed by exposure to 100 .mu.M glutamate for 42 h, lactate dehydrogenase levels within the cells remained at 62% of .mu.M untreated control values while glutamate-treated control fell to 0% lactate dehydrogenase. Betaine also exerted attenuating effects on N-methyl-D-asparte-, kainate-and quisqualate-induced neurotoxicity in a similar manner to that observed with glutamate. Similar neuroprotective effects of betaine with rat cortical cultures.
|
|
|
KEYWORD
|
|
|
Betaine, cultured chicken embryonic brain cells, Glutamate-induced neurotoxicity, Lactate dehydrogenase, Rat cortical cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|