|
KMID : 0043319960190030219
|
|
Archives of Pharmacal Research
1996 Volume.19 No. 3 p.219 ~ p.222
|
|
|
Elicitor-induced Phenylalanine-Ammonia Lyase, Cinnamic Acid 4-Hydroxylase and p-Coumaroyl transferase Activity in Ephedra Distachya Cultures
|
|
Song Kyung-Sik
Yutaka Ebizuka
|
|
|
Abstract
|
|
|
|
Ephedra olistachya cultures have been known to accumulate acids by elicitor treatment. Based on their chemical structures, the biosynthetic pathway ofacids was postulated and phenylalanine ammonia-lyase (PAL), cinnamic acid 4-hydroxylase (4-CH) and p-coumaroyl CoA: D-Ala p-coumaroyltransferase () were supposed to be involved in the pathway. The time course inductions of these enzymes were investigated after treatment of yeast extract, yeast-derived mannan glycopeptide and D-Ala. They were detectable at only 4 hours and reached to their maximum level at 9 hours after onset of elicitor treatment. The activities of PAL and 4-CH were almost disappeared within 24 hours, however, that of was remained up to 48 hours irrespective of the kind of elicitors. showed substrate specificity to D-Ala at crude enzyme extract level.
|
|
|
KEYWORD
|
|
|
Ephedra distachya, Tissue culture, Elicitor, p-Coumarylamino acids, Phenylalanine-ammonia lyase, Cinnacim acid 4-hydroxylase, p-Coumaroyl-transferase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|