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KMID : 0043319990220030232
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Archives of Pharmacal Research
1999 Volume.22 No. 3 p.232 ~ p.236
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Aloesin and Arbutin Inhibit Typrosinase Activity in a Synergistic Manner via a Different Action Mechanism
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Jin Ying Hua
Park Young-In
Cho Tae-Hyung
Lee Suk-Jin
Chung Myung-Hee
Park Jeong-Hill
Lee Seung-Ki
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Abstract
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In this study, we present evidence that cotreatment of aloesin and arbutin inhibits tyrosinase activity in a synergistic manner by acting through a different action mechanism. Aloesin or arbutin similarly inhibited enzyme activity of human- and mushroom-tyrosinases with an IC50 value of 0.1 or 0.04 mM, respectively. Lineweaver-Burk plots of the enzyme kinetics data showed that aloesin inhibited tyrosinase activity noncompetitively with a Ki value of 5.3 mM, whereas arbutin did it competitively (Maeda, 1996). We then examined whether cotreatment of these agents inhibits the tyrosinase activity in a synergistic manner. The results showed that 0.01 mM aloesin in the presence of 0.03 mM arbutin inhibited activity of mushroom by 80% of the control value and the reverse was also true. The inhibitory effects were calculated to be synergistic according to the B rgi method. Taken together, we suggest that aloesin along with arbutin inhibits in synergy melanin production by combined mechanisms of noncompetitive and competitive inhibitions of tyrosinase activity.
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KEYWORD
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Synergistic tyrosinase inhibitors, Aloesin, Arbutin
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