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KMID : 0043319990220030237
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Archives of Pharmacal Research
1999 Volume.22 No. 3 p.237 ~ p.242
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An Endogenous Proteinacious Inhibitor for S-Adenosyl-L-methionine-dependent Transmethylation Reactions; Identification of S-Adenosylhomocysteine as an Integral Part
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Seo Dong-Wan
Han Jeung-Whan
Hong Sung-Youl
Paik Woon-Ki
Lee Hyang-Woo
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Abstract
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A proteinacious inhibitor with a molecular weight of 1,600 Da which inhibits S-adenosyl-L-methionine-dependent transmethylation reactions was purified from porcine liver to homogeneity by procedures including boiling, Sephadex G-25 column chromatography and repeated HPLC. Employing both Nuclear Magnetic Resonance (NMR) and Fast Atom Bombardment-Mass (FAB-Mass) spectroscopy, S-adenosylhomocysteine was conclusively identified as an integral part of the inhibitor. The purified S-adenosylhomocysteine was competitive with S-adenosyl-L-methionine with Ki value of M towards protein methylase II.
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KEYWORD
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S-adenosylhomocystein, Methylation, Proteinacious inhibitor
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