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KMID : 0043319990220040367
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Archives of Pharmacal Research
1999 Volume.22 No. 4 p.367 ~ p.371
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Release of Renal Dipeptidase from Rabbit Renal Proximal Tubules and Its Inhibition by Gentamicin
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Kang Bok-Yun
We Jeoung-Soon
Choi Kyong
Lee Hwanghee-Blaise
Han Ho-Jae
Park Haeng-Soon
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Abstract
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Effects of several durgs on rabbit renal proximal tubules were examined for the applicability of renal dipeptidase (RDPase, EC 3. 4. 13. 11) release as a model system to study nephrotoxicity. The proximal tubule prepared by the method of Taub (1990) released RDPase spontaneously in the control experiment which was confirmed by Western blotting. RDPase was also released form cisplatin, lipopolysaccardie (LPS), and indomethacin-treated tubules. Gentamicin inhibited RDPase release in a concentration-dependent manner. This RDPase release system may not be a general model to screen nephrotoxicity but could be a useful source of RDPase purification in a simple and inexpensive way.
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KEYWORD
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Proximal tubule, Glycosylphosphatidylinositol (GPI)-protein, Renal dipeptidase (RDPase), Phosphatidyloinositol specific phospholipase C (PI-PLC), Gentamicin
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