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KMID : 0043319990220040404
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Archives of Pharmacal Research
1999 Volume.22 No. 4 p.404 ~ p.409
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Inhibitory Effects of Constituents of Gastrodia elata Bl. on Glutamate-Induced Apoptosis in MIR-32 Human Neuroblastoma Cells
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Lee Yong-Soo
Ha Jeoung-Hee
Yong Chul-Soon
Lee Dong-Ung
Huh Keun
Kang Young-Shin
Lee Sun-Hee
Jung Mi-Wha
Kim Jung-Ae
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Abstract
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The inhibitory effects of the constituents of Gastrodia elata Bl. (GE) on glutamate-induced apoptosis in human neuronal cells were investigated using IMR32 human neuroblastoma cells. Glutamate (GLU) induced DNA fragmentation, a hallmark of apoptosis, in a dose-dependent manner. GLU also induced a slow and sustained increase in intracellular concentration. Treatment with EGTA, an extracellular chelator, in a nominal -free buffer solution abolished the GLU-induced intracellular increase, indicating that GLU stimulated Ca2+ influx pathway in the IMR32 cells. BAPTA, an intracellualr chelator, significantly inhibited the GLU-induced apoptosis assessed by the flow cytometry measuring hypodiploid DNA content indicative of apoptosis, implying that intracellular rise may mediate the apoptotic action of GLU. Vanillin (VAN) and p-hydroxybenzaldehyde(p-HB), known constituents of GE, significantly inhibited both intracellular rise and apoptosis induced by GLU. These results suggest that the apoptosis-inhibitory actions of the constituents of GE may account, at least in part, for the basis of their antiepileptic activities. These results further suggest that intracelluarl signaling pathway may be a molecular target of the constituents of GE.
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KEYWORD
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Human neuroblastoma cells, Vanillin, p-Hydroxybenzaldehyde, Epilepsy, Glutamate, Apoptosis, Intracellular Ca2+
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