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KMID : 0043320060290070591
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Archives of Pharmacal Research
2006 Volume.29 No. 7 p.591 ~ p.597
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Artemisolide from Artemisia asiatica: Nuclear Factor-? (NF-?) Inhibitor Suppressing Prostaglandin E2 and Nitric Oxide Production in Macrophages
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Reddy Alavala Matta
Lee Jun-Young
Seo Jee-Hee
Kim Byung-Hak
Chung Eun-Yong
Ryu Shi-Yong
Kim Young-Sup
Lee Chong-Kil
Min Kyung-Rak
Kim Young-Soo
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Abstract
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Aerial parts of Artemisia asiatica (Compositae) have been traditionally used as an oriental medicine for the treatment of inflammatory and ulcerogenic diseases. In the present study, artemisolide was isolated as a nuclear factor (NF)-κB inhibitor from A. asiatica by activityguided fractionation. Artemisolide inhibited NF-κB transcriptional activity in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7 with an IC50 value of 5.8 μM. The compound was also effective in blocking NF-κB transcriptional activities elicited by the expression vector encoding the NF-κB p65 or p50 subunits bypassing the inhibitory kB degradation signaling NF-κB activation. The macrophages markedly increased their PGE2 and NO production upon exposure to LPS alone. Artemisolide inhibited LPS-induced PGE2 and NO production with IC50 values of 8.7 μM and 6.4 μM, respectively, but also suppressed LPS-induced synthesis of cyclooxygenase (COX)-2 or inducible NO synthase (iNOS). Taken together, artemisolide is a NF-κB inhibitor that attenuates LPS-induced production of PGE2 or NO via down-regulation of COX-2 or iNOS expression in macrophages RAW 264.7. Therefore, artemisolide could represent and provide the anti-inflammatory principle associated with the traditional medicine, A. asiatica.
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KEYWORD
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Artemisolide, Artemisia asiatica, Nuclear factor-κB, Prostaglandin E2, Nitric oxide, Macrophages
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