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KMID : 0043320080310050645
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Archives of Pharmacal Research
2008 Volume.31 No. 5 p.645 ~ p.652
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Suppression of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression by Tussilagone from Farfarae Flos in BV-2 Microglial Cells
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Lim Hyo-Jin
Lee Hyun-Sun
Ryu Jae-Ha
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Abstract
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Activated microglia produce diverse neurotoxic factors such as nitric oxide (NO) and prostaglandin that may cause neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. From the EtOAc soluble fraction of Farfarae flos (Tussilago farfara), we purified tussilagone as a bioactive compound by monitoring the inhibitory potential of NO production in activated microglia through the purification procedures. Tussilagone showed dose-dependent inhibition of NO and production in LPS-activated microglia with values of and , respectively. It suppressed the expression of protein and mRNA of inducible nitric oxide synthase and cyclooxygenase-2 through the inhibition of I- degradation and nuclear translocation of p65 subunit of NF-. Therefore tussilagone from Farfarae flos may have therapeutic potential in the treatment of neuro-inflammatory diseases through the inhibition of overproduction of NO and .
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KEYWORD
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Farfarae flos, Tussilagone, Nitric oxide, Nitric oxide synthase, Cyclooxygenase
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