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KMID : 0043320080310060805
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Archives of Pharmacal Research
2008 Volume.31 No. 6 p.805 ~ p.813
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Simultaneous Determination of Amphetamine-Type Stimulants and Cannabinoids in Fingernails by Gas Chromatography-Mass Spectrometry
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Kim Jin-Young
Cheong Jae-Chul
Kim Min-Kyoung
Lee Jae-Il
In Moon-Kyo
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Abstract
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A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (-tetrahydrocannabinol (-THC) and 11-nor--tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at for 30 min. The compounds of interest were isolated by liquidliquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, -THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients ( < 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails.
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KEYWORD
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Amphetamine-type stimulants, Cannabinoids, GC-MS, Fingernails
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