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KMID : 0043320080310070872
Archives of Pharmacal Research
2008 Volume.31 No. 7 p.872 ~ p.877
Cytoprotective Roles of Senescence Marker Protein 30 against Intracellular Calcium Elevation and Oxidative Stress
Son Tae-Gen

Kim So-Jung
Kim Keun-Ho
Kim Min-Sun
Chung Hae-Young
Lee Jae-Won
Abstract
Senescence marker protein 30 (SMP30) is identified as an important aging marker molecule and known to play multifunctional roles as an intracellular calcium regulatory protein in the signaling process. To elucidate the functional significance of SMP30, we established the stably transfected P19 cell line with SMP30 expression vector. Overexpression of SMP30 slightly suppressed the proliferation of P19 cells. However, SMP30 overexpression was cytoprotective against calcium-mediated stress such as calcium ionophore (A23187), and thapsigargin. We found that SMP30 overexpression reduced the elevated intracellular calcium levels induced by A23187, but not by thapsigargin. In addition, SMP30 transfected P19 cells were more protective to tert-butylhydroperoxide induced cytotoxicity, indicating the antioxidative properties of SMP30. Taken together, our results suggest that external calcium regulation and antioxidant properties are involved in the cytoprotective mechanism of SMP30.
KEYWORD
Aging, Calcium homeostasis, Endoplasmic reticulum, Oxidative stress, Plasma membrane, Senescence marker protein 30
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