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KMID : 0043320090320030453
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Archives of Pharmacal Research
2009 Volume.32 No. 3 p.453 ~ p.462
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Two Enone Fatty Acids Isolated from Gracilaria verrucosa Suppress the Production of Inflammatory Mediators by Down-regulating NF-κB and STAT1 Activity in Lipopolysaccharide-Stimulated RAW 264.7 Cells
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Yoo Eun-Sook
Park Sun-Soon
Yang Eun-Jin
Kang Gyeoung-Jin
Dang Hung-The
Lee Hye-Ja
Kang Hee-Kyoung
Jung Jee H.
Yoon Weon-Jong
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Abstract
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Gracilaria verrucosa is a common marine red alga that has anti-oxidant and anti-cancer properties. Recently, we reported that anti-inflammatory constituents of G. verrucosa operate through an unknown mechanism. For this reason, we isolated two enone fatty acids from G. verrucosa and investigated their molecular mechanism in LPS-stimulated RAW264.7 cells. We found that the two compounds inhibited the production of inflammatory markers (nitric oxide, TNF-α, and IL-6) in a dose-dependent manner. We next studied the effects of G. verrucosa compounds on LPS-induced signaling pathways. The two compounds suppressed NF-κB reporter activity by interfering with nuclear translocation of NF-κB and suppressed JAK/STAT (p-STAT1) signaling. These results suggest that G. verrucosa inhibits the production of inflammatory mediators (NO, TNF-α, and IL-6) by suppressing the activation of NF-κB and the phosphorylation of STAT1.
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KEYWORD
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Gracilaria verrucosa, Inflammation, Nitric oxide, Pro-inflammatory cytokines, NF-κB, STAT1
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