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KMID : 0043320090320060857
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Archives of Pharmacal Research
2009 Volume.32 No. 6 p.857 ~ p.862
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Bile Acid Derivatives from a Sponge-Associated Bacterium Psychrobacter sp.
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Jung Jee H.
Choi Sang-Ho
Hong Jong-Ki
Lee Chong-O.
Yoo Eun-Sook
Lee Hye-Ja
Pramod B. Shinde
Li Huayue
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Abstract
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In our search for bioactive metabolites from a marine sponge-associated bacterium Psychrobacter sp., a new bile acid derivative (1), which was assumed to be an artifact, were isolated along with six known (2-7) compounds by bioactivity-guided fractionation. Elucidation of the structure of the new compound was done using a combination of NMR (1H, 13C, HMBC, HSQC, and COSY) and MS spectroscopy. Compound 1 exhibited moderate suppressive effects on both NO and IL-6 production at a concentration of 200 μM (87.3 μg/mL) without significant cytotoxicity against cells. Compounds 2-5 and 7 showed selective inhibitory activity against several human pathogenic bacterial strains at the low concentration of 30 μg/well. In a cytotoxicity evaluation, only compound 7 showed mild cytotoxicity against five human solid tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT-15) with ED50 values in the range of 11-14 μg/mL.
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KEYWORD
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Sponge-associated, Psychrobacter sp., Bile acid, Anti-inflammatory, Antibacterial, Cytotoxicity
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