KMID : 0043320090320060857
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Archives of Pharmacal Research 2009 Volume.32 No. 6 p.857 ~ p.862
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Bile Acid Derivatives from a Sponge-Associated Bacterium Psychrobacter sp.
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Jung Jee H.
Choi Sang-Ho Hong Jong-Ki Lee Chong-O. Yoo Eun-Sook Lee Hye-Ja Pramod B. Shinde Li Huayue
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Abstract
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In our search for bioactive metabolites from a marine sponge-associated bacterium Psychrobacter sp., a new bile acid derivative (1), which was assumed to be an artifact, were isolated along with six known (2-7) compounds by bioactivity-guided fractionation. Elucidation of the structure of the new compound was done using a combination of NMR (1H, 13C, HMBC, HSQC, and COSY) and MS spectroscopy. Compound 1 exhibited moderate suppressive effects on both NO and IL-6 production at a concentration of 200 ¥ìM (87.3 ¥ìg/mL) without significant cytotoxicity against cells. Compounds 2-5 and 7 showed selective inhibitory activity against several human pathogenic bacterial strains at the low concentration of 30 ¥ìg/well. In a cytotoxicity evaluation, only compound 7 showed mild cytotoxicity against five human solid tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT-15) with ED50 values in the range of 11-14 ¥ìg/mL.
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KEYWORD
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Sponge-associated, Psychrobacter sp., Bile acid, Anti-inflammatory, Antibacterial, Cytotoxicity
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