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KMID : 0043320100330122017
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Archives of Pharmacal Research
2010 Volume.33 No. 12 p.2017 ~ p.2024
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Determination of the L-enantiomer of nateglinide in pharmaceutical formulations by micellar electrokinetic chromatography
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Phan Thanh Dung
Nguyen Ngoc Hoan
Kim Dae-Joong
Lee Yong-Jae
Choi Seung-Ho
Kim Kyeong-Ho
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Abstract
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An analytical micellar electrokinetic chromatographic method was developed and validated for the determination of the L-enantiomer of nateglinide. Separations were carried out in a 50 μm, 64.5/56 fused-silica capillary. The optimized conditions included 75 mM borate buffer, pH 9.2, containing 50 mM of sodium dodecyl sulfate and 25 mg/mL of methyl-β-cyclodextrin as background electrolyte, an applied voltage of 20 kV and a temperature of 15, UV detector at 210 nm. The assay was validated for the L-enantiomer of nateglinide. The limit of detection and quantification were 0.07 and 0.2% respectively. Intraday precision was ranged between 0.12 and 1.7%. Interday precision ranged between 0.73 and 1.73%. The assay was applied to the determination of the L-enantiomer of nateglinide in pharmaceutical formulations.
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KEYWORD
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Nateglinide, L-enantiomer, Micellar electrokinetic chromatography, Chiral separation, Methyl-β-cyclodextrin
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