|
KMID : 0043320110340050791
|
|
Archives of Pharmacal Research
2011 Volume.34 No. 5 p.791 ~ p.800
|
|
|
Induction of apoptosis by chelerythrine chloride through mitochondrial pathway and Bcl-2 family proteins in human hepatoma SMMC-7721 Cell
|
|
Zhang Zheng fu
Guo Ying
Zhang Jian bin
Wei Xiong hui
|
|
|
Abstract
|
|
|
|
The objective of this study was to evaluate the antitumor activity of chelerythrine chloride (CHE) and investigate its potential apoptotic induction mechanism in SMMC-7721 cells. Our results suggested that the proliferation of SMMC-7721 cells was inhibited by CHE in a time and dose dependent manner, with a significant accumulation in S phase, and the cells exhibited typical apoptotic features. Moreover, CHE remarkably induced apoptosis by disruption of the mitochondrial membrane potential, release of Cyt-c, activation of caspase-3, and cleavage of poly-ADP-ribose polymerase in a dose dependent manner. Furthermore, the expression of Bcl-xl was downregulated while Bax and Bid expression was upregulated, and no variation was found for Bcl-2. These results indicated that CHE may play an important role in suppression of tumor growth by inducing apoptosis in human hepatoma cells via the activation of a mitochondrial pathway and regulating the expression of Bcl-2 family proteins.
|
|
|
KEYWORD
|
|
|
Chelerythrine chloride, Proliferation, Apoptosis, Human hepatoma SMMC-7721, Mitochondrial pathway, Bcl-2 family proteins
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|