|
KMID : 0043320110340081339
|
|
Archives of Pharmacal Research
2011 Volume.34 No. 8 p.1339 ~ p.1345
|
|
|
Effect of saucerneol D on melanin production in cAMP-elevated melanocytes
|
|
Yun Ji-Young
Roh Eun-Mi-Ri
Son Jong-Keun
Lee Seung-Ho
Seo Chang-Seob
Hwang Bang-Yeon
Han Sang-Bae
Kim Young-Soo
|
|
|
Abstract
|
|
|
|
Intracellular cAMP stimulates microphthalmia-associated transcription factor (MITF) induction in melanocytes through cAMP-responsive element binding protein (CREB), which plays a pivotal role in the gene expression of tyrosinase for melanin biosynthesis. In the present study, saucerneol D as a lignan constituent of Saururus chinensis (Saururaceae family) efficiently inhibited melanin production with IC50 values of 188?297 nM in B16 melanoma cells stimulated with α-melanocyte stimulating hormone (α-MSH) or other cAMP elevators. Moreover, saucerneol D down-regulated α-MSH-induced gene expression of tyrosinase at the transcription level in B16 cells, but it did not directly inhibit the catalytic activity of cell-free tyrosinase. As to the molecular basis of hypopigmenting action, saucerneol D inhibited α-MSH-induced phosphorylation of CREB in the cells, and sequentially suppressed MITF induction. Taken together, this study provides saucerneol D down-regulated the gene expression of tyrosinase, resulting in the inhibition of cAMP-induced melanin biosynthesis, and suggests pharmacological potential of the lignan structure in skin hyperpigmentation.
|
|
|
KEYWORD
|
|
|
Saucerneol D, Lignan structure, Hypopigmenting activity, Tyrosinase expression, MITF induction
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|