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KMID : 0043320180410020219
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Archives of Pharmacal Research
2018 Volume.41 No. 2 p.219 ~ p.228
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Comparison of the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide on Japanese encephalitis vaccine in mice
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Ko A-Ra
Wui Seo-Ri
Ryu Ji-In
Do Hien Thi Thu
Lee Yeon-Jeong
Lim Soo-Jeong
Rhee In-Moo
Jung Dae-Im
Park Jin-Ah
Choi Jung-Ah
Song Man-Ki
Lee Na-Gyong
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Abstract
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Adjuvants are essential vaccine components used to enhance, accelerate, and/or prolong adaptive immunity against specific vaccine antigens. In this study, we compared the adjuvanticity of two adjuvant formulations containing de-O-acylated lipooligosaccharide (dLOS), a toll-like receptor 4 agonist, on the Japanese encephalitis (JE) vaccine in mice. Mice were immunized once or twice at a two-week interval with inactivated JE vaccine in the absence or presence of adjuvant. We found that both the alum- and the liposome-based formulation induced significantly faster and higher serum IgG antibody responses as compared with the non-adjuvanted vaccine after either one or two immunizations. The antibody titers of the mouse immune sera correlated with 50% plaque reduction neutralization test (PRNT50) antibody titers. In addition, the dLOS/liposome formulation was more effective in inducing a Th1-type immune response than the dLOS/alum formulation, as suggested by a strong antigen-specific interferon (IFN)-γ response. Based on these results, we suggest that both alum- and liposome-based adjuvant formulations containing dLOS may be used for the development of JE vaccines with improved immunogenicity.
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KEYWORD
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Adjuvant formulation, De-O-acylated lipooligosaccharide, Japanese encephalitis vaccine
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