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KMID : 0350519930460041531
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1531 ~ p.1542
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The Myocardial Protective Effect of Superoxide Dismutase on Ischemic-reperfusion injury in Isolated Rabbit Heart
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Abstract
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Reperfusion damage refers to the serious structural, functional, and metabolic derangements commonly observed during restoration of coronary circulation following cardiopulmonary bypass. Damage is believed to result from ischemic injury incurred
during
aortic cross-clamping and unmasked in the recovery period and certain metabolic process is activated during post-ischemic reperfusion.
Many studies have tested a variety of cardioplegic solution composition and administration modes. However, much controversy exisis over the different methods. In isolated rabbit heart, we tested the hypothesis that improved myocardial protection
during
cardioplegia can prevent ischemicreperfusion damage and investigated the possibility of achieving optimal myocardial protection. Elective cardiac arrests were induced in isolated perfused rabbit hearts under working conditions. We tested two
methods of
cardioplegic soltion, one conventional cold hyperkalemic crystalloid solution(n=7) and another supcroxide dismutase(SOD) added cold one(N=7). Hemodynamic functional recovery, the concentration of creatine kinase detected in coronary effluent, and
the
structural findings were used to evaluate the advantage of SOD added cardioplegic solution.
@ES The results were as follows ;
@EN 1. The SOD in cardioplegic solution enhanced recovery of aortic flow when compared with control group, the percentage recovery after 90 minutes´ ischemia being 85.1±5.7% in SOD-treated group and 76.1±6.2% in control group(P<0.05).
2. The increase in concentration of creatine kinase was less in SOD-treated group compared with control group(48.1±5.8 I.U./L vs 74.7±7.2 I.U./L, P<0.05).
3. The transmission electron microscopic findings revealed more mitochondrial swelling, more depletion of glycogen granules, and some amorphous matrix densities in control group compared with SOD-treated group.
We concluded that good tolerance to global ischemic cardiac arrest could be achieved when superoxide dismutase was used as the additive to the cardioplegic solution.
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