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KMID : 0350519940470031301
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Journal of Catholic Medical College
1994 Volume.47 No. 3 p.1301 ~ p.1312
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The Effects of Multi-lamellar Vesicles on Lipids Barrier Disruption in Hairless Skin:An Evaluation of the Changes of Stratum Corneum Intercellular lamellar Bilayers by the Electronmicroscopy
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Abstract
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The intercellular lamellar bilayers are very important for stratum corneum barrier function, however the knowledge about the structure of these bilayers is limited due to poor visualization and retention of them. The substitution of ruthenium
tetroxide(RuO4) fixation for osmium tetroxide(OsO4) is currently employed to assess these lipid bilayers. In order to investigate the effects of multi-lamellar vesicles analogous to lamellar structure of stratum corneum lipids on the disruption
of
stratum corneum lipid barrier induced by acetone, the morphologic changes of stratum corneum lipids were evaluated in the hairless mice skin by electronmicroscopy under the various conditions.
Twenty severn hairless mice weighing 20-30g were divided into 3 group ; normal control(group 1, n=3), the air exposed group(group 2. N=12), and the parafilm-occluded group(group 3, n=12). The air exposed group(group 2) was subdivided into 3
group:
acetone applied group(group 2A, n=4), acetone and multi-lamellar vesicles applied group(group 2B, n=4) and acetone and vehicle applied group(group 2C, n=4). Also, parafilm-occluded group was subdivided into 3 group(group 3A, 3B and 3C) as in
group
2.
Skin biopsies were taken from the mice of each subgroups for the electronmicroscopic studies at 1hr, 3hr, 6hr, and 24hr post-treatments.
@ES The results were as follows;
@EN 1. In the air-exposed group, the intercellular lamellar bilayers were visualized rapidly and much more in group 2B(acetone and multi-lamellar vesicles applied group) than in group 2A(acetone applied group).
2. In the parafilm-occluded groups, we found the less severe degree of seperation of the layers of stratum corneum and the more lamellar bilayer in group 3B(acetone and multi-lamellar vesicles applied group) in comparison with group 3A(aetone
applied
group).
3. When we compared group 3A, 3B, 3C with 2A, 2B, 2C in terms of a tendency to formation of intercellular lamellar bilayers, the parafilm-occluded groups(group 3A, 3B and 3C) had a tendency to slower and lesser formation of intercellular
lamellar
bilayers than the air exposed groups(group 2A, 2B and 2C).
In conclusion, we could visualize the stratum corneum intercellular lamellar bilayers and lamellar bodies by the electronmicroscope using ruthenium tetroxide fixation method and the above-mentioned results suggest that topical application of
multi-lamellar vesicles may repair the disruption of skin surface lipid barrier and pormote the synthesis of stratum corneum lipid bilayers after the exposure to acetone.
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