The study was carried out for comparison of variants and development of genetic markers using Randomly Amplified Polymorphic DNA(RAPD) analysis method. The ginseng variants used were as follows; Chungkyung-Chong, Hwangskoog-Chong, KG101 selected
by
the
pure-line selection method and 6 kinds of Jakyung-Chong strains (Jinjakyung, Jakyung-Chong 31783, Jakyung-Chong 847913, Jakyung-Chong 79742, Jinjakyung of USSR, and Mimaki of Japan).
Four of 10 RAPD primers showed the distinctive polymorphism among 9 ginseng variants and lines, and were selected for more detailed polymorphic analysis. The sequences of 4 selected primers were TRGCGAGCTG (primer#2), AATCGGCTG (#4), GAAACGGGTG
(#7),
and GTGACGTAGG (#8). All primers produced several common bands among the strains. However, when primer #2 was applied, the electrophoregram showed the specific band at 1.8kb region in Chungkyung-Chong, Hwangskoog-Chong, and KG101, and 1 kb in the
Jakyung-Chong 847913. In primer #4, 1.1 kb band was shown in Chungkyung-Chong, Hwangskoog-Chong, KG101 and Jakyung-Chong 79742. In primer #7, 700bp band only in Mimaki, comparing to another strains.
When Similarity Index (XI) Was calculated, Chungkyung-Chong and Hwangskoog-Chong, and Jakyung-Chong 81783 and Jinjakyung of USSR showed the most close SI, 0.11 and 0.08 respectively. The data of KG101. Which showed the SI of 0.13 with the group
of
Chungkyung-Chong and Hwangskoog-Chong, coincided with the fact that it was released from Hwangskoog-Chong by breeding process. The data of Jakyung strains indicated the significant variation among the strains. Prom these results, RAPD analysis
method
could be succesively applied to the classification and genetic analysis for breeding of Korean ginseng.
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