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KMID : 0352720070310020086
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Journal of Ginseng Research
2007 Volume.31 No. 2 p.86 ~ p.92
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Effect of Korean red ginseng on collagen biosynthesis and MMP-I activity in human dermal fibroblast
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Kim Na-Mi
Hwang Eui-Il
So Seung-Ho
Do Jae-Ho
Koo Bon-Suk
Lee Seong-Kye
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Abstract
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This study was carried out to develop health & functional food by using Korean red ginseng for prevention of skin wrinkles. Effects of Korean red ginseng on the collagen biosynthesis and inhibition of matrix metalloproteinase-I (MMP-1) activity in human dermal fibroblast were investigated. Crude saponin contents of Korean red ginseng water extract (WE), Korean red ginseng ethanol extracts (EE) and Korean Red ginseng purified extracts (PE) were 72 ㎎/g, 107 ㎎/g and 220 ㎎/g, respectively. We incubated human fibroblast cell with Korean red ginseng component by addition of 1 ㎍/㎖, 5 ㎍/㎖, 10 ㎍/㎖. Amount of collagen biosynthesis was 1.86 ng/㎖ in control sample and 2.85 ng/㎖, 2.05 ng/㎖ and 2.58 ng/㎖ in retinoic acid, EE and PE respectively. Furthermore, ginsenoside-Rg₁ and ginsenoside-Rb₁ were shown 2.01 ng/㎖ and 3.07 ng/㎖. MMP-1 activities of EE, PE, ginsenoside-Rg₁ and ginsenoside-Rb₁ were decreased to 92%, 94%, 91% and 78% respectively as compared with control. Cell proliferation were showed 84-96% in the Korean red ginseng components. The antioxidative SOD activities of the Korean red ginseng components were showed 28-69%, however it was lower than that of Vitamin C. From this results, we conclude that Korean red ginseng have a anti-wrinkle effect and ginsenoside-Rb₁ may be considered as a more effective component.
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KEYWORD
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human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I, skin health foods, Korean red ginseng
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