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KMID : 0352720210450020305
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Journal of Ginseng Research
2021 Volume.45 No. 2 p.305 ~ p.315
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Identification and quantification of oleanane triterpenoid saponins and potential analgesic and anti-inflammatory activities from the roots and rhizomes of Panax stipuleanatus
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Shu Pan-Pan
Li Lu-Xi
He Qin-Min
Pan Jun
Li Xiao-Lei
Zhu Min
Yang Ye
Qu Yuan
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Abstract
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Background: Panax stipuleanatus represents a folk medicine for treatment of inflammation. However, lack of experimental data does not confirm its function. This article aims to investigate the analgesic and anti-inflammatory activities of triterpenoid saponins isolated from P. stipuleanatus.
Methods: The chemical characterization of P. stipuleanatus allowed the identification and quantitation of two major compounds. Analgesic effects of triterpenoid saponins were evaluated in two models of thermal- and chemical-stimulated acute pain. Anti-inflammatory effects of triterpenoid saponins were also evaluated using four models of acetic acid?induced vascular permeability, xylene-induced ear edema, carrageenan-induced paw edema, and cotton pellet?induced granuloma in mice.
Results: Two triterpenoid saponins of stipuleanosides R1 (SP-R1) and R2 (SP-R2) were isolated and identified from P. stipuleanatus. The results showed that SP-R1 and SP-R2 significantly increased the latency time to thermal pain in the hot plate test and reduced the writhing response in the acetic acid?induced writhing test. SP-R1 and SP-R2 caused a significant decrease in vascular permeability, ear edema, paw edema, and granuloma formation in inflammatory models. Further studies showed that the levels of inflammatory mediators, nitric oxide, malondialdehyde, tumor necrosis factor-α, and interleukin 6 in paw tissues were downregulated by SP-R1 and SP-R2. In addition, the rational harvest of three- to five-year-old P. stipuleanatus was preferable to obtain a higher level of triterpenoid saponins. SP-R2 showed the highest content in P. stipuleanatus, which had potential as a chemical marker for quality control of P. stipuleanatus.
Conclusion: This study provides important basic information about utilization of P. stipuleanatus resources for production of active triterpenoid saponins.
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KEYWORD
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Analgesic, Anti-inflammatory, Panax stipuleanatus, Paw edema, Stipuleanosides
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