|
KMID : 0354720070310030230
|
|
Journal of Korean Diabetes Association
2007 Volume.31 No. 3 p.230 ~ p.235
|
|
|
Effects of Lovastatin on Free Fatty Acid Oxidation in Cultured L6 Rat Skeletal Muscle Cells
|
|
Kim Dong-Lim
Song Kee-Ho
Kim Hae-Rim
Kim Suk-Kyeong
|
|
|
Abstract
|
|
|
Background: Recent clinical studies suggest that statins improve insulin resistance and glucose metabolism
in patients with metabolic syndrome and type 2 diabetes. To evaluate the possible mechanism of this action, we measured free fatty acid oxidation in cultured L6 rat skeletal muscle cell line.
Methods: Cultured L6 myotubes were treated with or without lovastatin (1, 5, 20 μM) for 24 hours or 48 hours and palmitate oxidation was measured. We also measured protein concentration of the cells.
Results: Lovastain increased palmitate oxidation in dose and time dependent manner in L6 myotubes (24 hr;
1 μM 119.2 ± 11.9% of control, 5 μM 140.9 ± 8.1%, 20 μM 150 ± 5%, P = 0.05 vs control, respectively, 48 hr 1 μM 120.9 ± 14.5%, 5 μM 176.6 ± 28.2%, 20 μM 196.0 ± 19.9%, P < 0.01 vs control, respectively). However, lovastatin decreased total cellular protein (24 hr: 1 μM 89.2 ± 6.1% of control, 5 μM 79.3 ± 7.6%, 20 μM 65.4 ± 4.2%, P = 0.05 vs control, respectively, 48 hr: 1 μM 81.7 ± 5.1%, 5 μM 58.6 ± 11.9%, 20 μM 48.1 ± 6.9%, P < 0.01 vs control, respectively).
Conclusion: Lovastatin increased skeletal muscle free fatty acid oxidation in L6 rat skeletal muscle cells. This
would be one of the mechanisms which lovastatin improves insulin resistance.
|
|
|
KEYWORD
|
|
|
Fatty acid oxidation, Skeletal muscle, Statins
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|