KMID : 0358920150420010010
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Journal of the Korean Academy of Pedodontics 2015 Volume.42 No. 1 p.10 ~ p.21
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Detection of Streptococcus mutans in Saliva using Monoclonal Antibodies
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Lee Min-Jeong
Lee Daw-Woo Kim Mi-Ah Lee Kyung-Yol Baik Byeong-Ju Yang Yeon-Mi Kim Jae-Gon
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Abstract
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The purpose of this study was to determine the usefulness of a detection method for Streptococcus mutans in saliva with monoclonal antibodies developed targeting Ag I/II and glucosyltransferases (gtf B, gtf C and gtf D) in Streptococcus mutans. In the three groups tested (adults, minors, and minors under orthodontic treatment), the results of the DMFT scores, the colony density (CFU/mL) in their saliva was measured using Dentocult??-SM strip mutans, polymerase chain reaction was performed to test whether Streptococcus mutans and Streptococcus sobrinus were present, and Streptococcus mutans detecting tests performed in their saliva using four types of monoclonal antibody were collected. In conclusion, it was demonstrated that the Streptococcus mutants plays more important role in forming dental caries compared to Streptococcus sobrinus, and that the monoclonal antibodies against glucosyltransferases (gtf B, gtf C, gtf D) and Ag I/II of Streptococcus mutans are superior in detecting Streptococcus mutans to Dentocult??-SM strip mutans or polymerase chain reaction
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KEYWORD
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Monoclonal antibody, Streptococcus mutans, Glucosyltransferase B, C, D, Ag I/II
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