KMID : 0359319980380010139
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Korean Journal of Veterinary Research 1998 Volume.38 No. 1 p.139 ~ p.145
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In vitro isolation of a bovine Neospora in Korea
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±èÀçÈÆ/Kim, Jae Hoon
¼ÕÇöÁÖ/±è´ë¿ë/Ȳ¿ì¼®/À̺´Ãµ/ȲÀÇ°æ/Áø¿µÈ/Sohn, Hyun Joo/Kim, Dae Yong/Hwang, Woo Suk/Lee, Byung Chun/Hwang, Eui Kyung/Jean, Young Hwa
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Abstract
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The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.
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