KMID : 0359320170570030201
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Korean Journal of Veterinary Research 2017 Volume.57 No. 3 p.201 ~ p.204
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Isolation and identification of Moraxella cuniculi from a rabbit with keratoconjunctivitis
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Yang Dong-Kun
Kim Ha-Hyun Yoo Jae-Young Lim Suk-Kyung Yoon Soon-Seek Cho In-Soo
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Abstract
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A Gram-negative, catalase- and oxidase-positive, coccus-shaped bacterium was isolated from a rabbit with keratoconjunctivitis. Colonies of the isolate were round, smooth, and exhibited hemolytic activity on 5% sheep blood agar. Scanning electron microscopy revealed 0.4 to 0.5 ¥ìm diameter oval cocci. Partial 16S rRNA gene (1446 bp) sequence analysis demonstrated the isolate had significant homology with the Moraxella cuniculi CCUG2154 strain isolated from a rabbit in Germany in 1973. Our isolate was designated as APQAB1701. Antibiotic susceptibility tests demonstrated that APQAB1701 was sensitive to 24 antibiotics; 3 of the antibiotics (nalidixic acid, spectinomycin, and colistin) had minimal inhibitory concentrations ¡Ã 32 ¥ìg/mL against the isolate.
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KEYWORD
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Moraxella cuniculi, identification, isolation, rabbits
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