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KMID : 0361319870190010156
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Korean Journal of Medical Technologists
1987 Volume.19 No. 1 p.156 ~ p.160
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Study of Toluidine Blue O-Mediated Techniques for Dnase Detection
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Abstract
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The TBO studies were performed with various concentrations of dye to determine the optimal concentration for both bacterial growth and detection of DNase activity.
1 . V. vulnificus no discernible indicating DNase activity were evident from all concentration TBO.
V. parahaemolyticus showed pinkzone of TBO from 0.1%--0.025% DNase activity exhibited by 0.075% and 0.05% TBO were at maximal levels for V. parahaemolyticus DNase-positive organism. A. hydrophila showed pinkzone of TBO from 0.25% to 0.005% when the 0.005% TBO solution was used, DNase activity was difficult to observe because the colors were too faint.
2. Effect of TBO on bacterial growth.
A. hydrophila showed very little or no inhibition of growth at any concentration of TBO from 0.5%--0.005%. V. parahaemolyticus was inhibited completely when dye concentrations exceeded 0.075% but showed no inhibition in the presence of 0.025% TBO. V. vulnificus was inhibited completely when dye concentrations exceeded 0.025% but showed no inhibition in the presence of 0.01% TBO.
3 . Detection of DNase activity with TBO in coporated in to the DNase test agar affected the ability to detect DNase ability.
V. vulnifzcus growth when dye concentration of TBO from 0.01% to 0.005% but no discernible pinkzones indicating DNase activity were evident. V. parahaenwlyticus showed positive reaction significantly on TBO containing DNase agar. from 0.05% to 0.005%.
The production of DNase by V. parahaemolyticus was partially masked on plates containing 0.05% TBO. A. hydrophila showed positive react significant on from 0.25% to 0.005% TBO containing DNase agar.
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