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KMID : 0362720230610040276
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The Journal of the Korean Dental Association
2023 Volume.61 No. 4 p.276 ~ p.284
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Effect of phosphatase and tension homolog (PTEN) on periodontal complex
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Kim Ye-Hyun
Choi Won-Jun
Ni Xiong
Lim Won-Hee
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Abstract
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Purpose: Phosphatase and tension homolog (PTEN) has been known to play a role of maintenance of homeostasis over the body. It is little known about the roles of PTEN in periodontal complex. The purpose of this study was to explore the role of PTEN in periodontal complexes after removing PTEN from osteoblasts and odontoblasts.
Materials and Methods: The resulting OCN?Cretg/+;Ptenfl/fl mice were created after by conditionally eliminating the PTEN gene using an Osteocalcin (OCN) Cre driver and the resulting OCN?Cretg/+;Ptenfl/fl mice (experimental group) were compared with the wild type mice (control group) using micro-CT, histology and immunohistochemical analyses. The amount of dentin and alveolar bone was analyzed, and osteopontin and bone sialoprotein in the periodontal ligament space (PDL) were evaluated and collagen in the PDL was analyzed quantitatively and qualitatively.
Results: Micro CT showed that dentin and bone were significantly accumulated in an experimental group. Dentin sialoprotein was significantly increase in dentin of an experimental group. Internal balance in the PDL was altered with increased expression of PCNA in an experimental group.
Conclusions: Loss of PTEN causes an increase of dentin and bone in the periodontal complexes with an alteration of internal balance in the PDL. These findings underscore the effect of PTEN on homeostatic control of periodontal complexes.
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KEYWORD
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PTEN, homeostasis, periodontal complex
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