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KMID : 0363319990200010099
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Journal of Korean Oriental Internal Medicine
1999 Volume.20 No. 1 p.99 ~ p.110
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Effects of Chiyangtang on Helicobacter pylori-induced increase of cytokines gene expression
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Lee Hyung-Ju
Won Jin-Hee
Moon Goo
Moon Seok-Jae
Park Dong-Won
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Abstract
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Effects of Chiyangtang(CYT) on H. pylori-induced increase of interleukin 8 and interleukin 1 gene expression was studied in Kato Ⅲ cell line, a human stomach epithelial cell line. Treatment of H. pylori to the cell culture signifant!y increased IL-8 and IL-1 mRNA synthesis. When CYT was added along with H. pylori, the increase of IL-8 and IL-1 mRNA synthesis was blocked. Activation of transcription factor and AP-1 which were known to important in IL-8 and IL-1 gene expression was also studied using chloramphenicol acetyltransferase(CAT) assay. Treatment of H. pylori increased activation of and AP-l and CYT effectively protected the activation. Electrophoretic mobility shift assay suggested that CYT effectively inhibited DNA binding of and AP-l to their cognate site.
These results suggested that CYT could prevent stomach diseases through the down regulation of IL -8 and IL-l gene expression which might be mediated by the inhibition of and AP-1 activities and their binding to DNA.
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KEYWORD
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Helicobacter pylori, Chiyangtang, IL-8, IL-1, NF-, AP-1
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