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KMID : 0364219790220030115
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Korean Journal of Zoology
1979 Volume.22 No. 3 p.115 ~ p.124
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Subunit Interactions of Vertebrate Lactate Dehydrogenase: I. Immunochemistry of Subunits
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Park Sang-Yoon
Yum Jung-Joo
Kim Sang-Yeop
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Abstract
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Two homotetrameric lactate dehydrogenase isozymes from Fluta alba and one from Ophicephalus argus were purified by combination of gel filtration and DEAE-cellulose chromatogrphy. The final preparations were isozymically pure and used to elicit antibodies in rabbits. The immunochemical reactivities demonstrated that the amino acids of active site is not to be included in the antigenic determinants, that antibodies or unknown component of immunized rabbit serum might be responsible for the electrophoretic abnormality and that two subunits share common antigenic determinants, reflecting that these polypeptides have a common evolutionary origin.
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