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KMID : 0364219910340020209
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Korean Journal of Zoology
1991 Volume.34 No. 2 p.209 ~ p.216
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Effect of Insulin on Differention of Chick Embryonic Neuroblasts Cultured in vitro
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Lee Chang-Ho
Choi Deog-Young
Park Hye-Gyeong
Kwak Kyu-Bong
Kim Hye-Sun
Chung Chin-Ha
Ha Doo-Bong
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Abstract
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To examine the effed of Insulin on neuronal differentiation, telencephalic neuroblasts from chick embryonic brains were cultured in a serum-free medium. Indirect immunofluorescence microscopic studies revealed that the spedfic protein, MAP-2, was localized in both cell bodies and neurites of developing neuroblasts. Furthermore, treatinent of increasing concentration of Insulin promoted the MAP-2 synthesis as well as the neurite outgrowth activity. Thus, the enhancement of the morphological and biochemical parameters for neuronal differentiation appears to he closely correlated, and the neurotrophic effect of insulin may play a crucial role in neuronal process formation.
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KEYWORD
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Telencephalon, Insulin, Neurite outgrowth, MAP-2
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