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KMID : 0364819880260020082
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Korean Journal of Microbiology
1988 Volume.26 No. 2 p.82 ~ p.87
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Genetic analysis of protoplast fusants of Candida pseudotropicalis
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Abstract
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1
The genetic analysis and characterization of protoplast fusion hybrids between complemeatary auxotrophic mutants of Candida pseudotropicalis were carried out. Nuclear fusion appeared to occur in fusion hybrids (e.g., F15 and F33), as strongly suggested by isolation of recombinants after mitotic segregation of parental genetic markers. This was confirmed by DNA content, unclear staining and comparison of survival rate to UV light. After keeping fusion hybrids for approximately out year, the frequency of spontaneous mitotic segregation was 3.0 x 10-4-g.1 x 10-4 while that of induced mitotic segregation was 1.4 x 10-3-1.7 x 10-3. These results suggested that they maintained stable karyogamy state. It was also found that the production of P-D-galactosidase from F15, F33 and F158 was somewhat increased when compared with that from either anxotrophic parents or wild type.
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KEYWORD
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