|
KMID : 0364819940320040280
|
|
Korean Journal of Microbiology
1994 Volume.32 No. 4 p.280 ~ p.284
|
|
|
A possible role of lipopolysaccharides in the prevention of lysosome0symbiosome fusion as studied by microinjection of an anti-LPS monoclonal antibody
|
|
최의열:Choi Eui-Yul
저자없음:No authors listed
|
|
|
Abstract
|
|
|
|
Lack of lysosomal fusion with symbiosome in symbiot0bearing Amoeba proteus may be due either to the presence of a component in the symbiosome membrane or to the absence of a component needed in the fusion process. Using monoclonal antibody as a probe, lipopolysaccharides were identified as symbiosome-membrane components contributed by symbionts and were found to be exposed on the cytoplasmic side of the membrane. In order to test whether lipopolysaccharided antibody was microinjected and processed for double immunostaining in conjunction with anti-lysosome antibody as a lysosome-fusion indicator. Microinjection of the anti-LPS antibody caused symbiosomes to fuse with lysosomes, suggesting that X-bacterial lipopolysaccharided could be ‘fusion-preventing’ factors.
|
|
|
KEYWORD
|
|
|
Monoclonal antibodies, microinjection, lysosome fusion, lipopoysaccharides, symbiosis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|