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KMID : 0364820090450010058
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Korean Journal of Microbiology
2009 Volume.45 No. 1 p.58 ~ p.62
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Identification and Cultural Characterization of Lipase Production Bacteria Isolated from
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Kim Man-Chul
Yeo In-Kyu
Jang Ik-Soo
Heo Moon-Soo
Jang Tae-Won
Ramasamy Harikrishnan
Jeong Joon-Bum
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Abstract
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From the course of screening of useful enzyme producing microorganism from marine sedimentary layer, we isolated 2 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram-positive bacteria grouped on Janibacter sp. An excellent lipase producing strain, Janibacter sp. LI-68 and J. sp. LI-80 identified by 16S rDNA analysis and biochemical methods (BIOLOG), was further studied its lipase producing characteristics. The optimum initial pH, temperature and the optimum cultral time for the enzyme production on MA medium were 8, 30~40oC and 96 h, respectively.
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KEYWORD
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16S rDNA, Janibacter sp., lipase, marine sedimentary layer
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