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KMID : 0364820090450010082
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Korean Journal of Microbiology
2009 Volume.45 No. 1 p.82 ~ p.85
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Biodegradation of Endocrine Disrupting Chemicals by Genetic Transformants of Phlebia
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Kum Hyun-Woo
Kim Myung-Kil
Choi Hyung-Tae
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Abstract
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Endocrine disrupting chemicals (EDCs) disturb animal hormonal system even at very low concentrations, and finally give harmful effects to human through the food web. A white rot fungus Phlebia tremellosa isolated in Korea, was reported to have good degrading activity against the endocrine disrupting phthalates. However, this fungus has very low manganese peroxidase (MnP) activity under various culture conditions while laccase and lignin peroxidase activities were high. We have isolated an MnP cDNA from Trametes versicolor which was involved in the degradation of EDCs, and constructed an MnP expression vector to use in the genetic transformation of P. tremellosa in order to get higher MnP producing strains. Many transformants had integrated expression vector in their chromosomal DNAs, and showed increased MnP activity. One of two transformants showed increased degradation of 4 EDCs (70~88%) than the wild type (30~45% degradation rates), and showed twice better removal of estrogenic activities generated by the EDCs than the wild type.
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KEYWORD
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endocrine disrupting chemicals, estrogenic activity, manganese peroxidase, Phlebia tremellosa
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