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KMID : 0364820100460020148
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Korean Journal of Microbiology
2010 Volume.46 No. 2 p.148 ~ p.151
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Analysis of Genotype and Phenotype of Erythromycin Resistance in Enterococci spp. Isolated from Raw Milk Samples
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Lee Hye-In
Jung Jae-Hyuk
Lee Sang-Jin
Choi Sung-Sook
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Abstract
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The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (≥64 mg/ml). Among 110 isolates, 86.3% (n=95) showed cMLSB phenotype and 13.6% (n=15) showed iMLSB phenotype. All of isolates have erm(B) determinant, 75.45% (n=83) have mef(A) an efflux system determinant. The majority of Enetrcococci isolated from raw milk samples in northern area of Gyeonggi-Do showed high level of resistance to erythromycin.
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KEYWORD
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enterococci, erythromycin resistance, genotype, phenotype
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