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KMID : 0368420130560030168
Journal of Plant Biology
2013 Volume.56 No. 3 p.168 ~ p.175
Characterization of the first tuber mustard calmodulin-like gene, BjAAR1, and its functions in responses to abiotic stress and abscisic acid in Arabidopsis
Xiang Liuxin

Xia Yuxian
Cai Yingfan
Liu Jijun
He Xiaohong
Sun Quan
Wang Xiaoyan
Fu Yuyin
Fan Yonghong
Dong Daiwen
Zhou Guanfan
Shen Jinjuan
Liu Yihua
Abstract
The first tuber mustard calmodulin-like (CML) gene BjAAR1 (Brassica juncea var. tumida Tsen et Lee Abiotic stress and Abscisic acid (ABA) Responsive gene 1) was cloned and characterized. The protein encoded by BjAAR1 contains four predicted Ca2+ binding sites (EF-hand motif) and its recombinant protein can bind Ca2+ in vitro. qRT-PCR showed that the expression level of BjAAR1 was rather high in non-swollen stem of tuber mustard and largely reduced in swollen stem. Expression of BjAAR1 enhanced ABA- and stress-induced gene expression in Arabidopsis (Arabidopsis thaliana). Transgenic plants also exhibited hypersensitivity to NaCl, mannitol, and ABA during the seed germination and post-germination stages. ABA biosynthesis inhibitor, norflurazon (NF), rescued hypersensitivity phenotype of transgenic plants to NaCl and mannitol, indicating that BjAAR1 functions in multiple abiotic stresses response through ABA-dependent process.
KEYWORD
ABA, Abiotic stress, Arabidopsis, BjAAR1, Calmodulin-like gene, Tuber mustard
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